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Immunological Experiments with Denatured and Insoluble Proteins

From the Department of Biochemistry and Pharmacology of the University of California, Berkeley

Abstract

To study the influence of denaturation, coagulation and insolubility on the production of fixation antibodies, rabbits were immunized respectively against the following antigens: Egg albumin heated (dry) to 110°C., egg albumin heated in solution, to 100°C. without coagulation, egg albumin coagulated by heat. The sera of these animals reacted equally with the native (unheated) and with the denatured antigen. Using sodium caseinate as antigen, fixation antibodies were demonstrated in the sera of rabbits which had been immunized by injecting a suspension of washed casein. The theoretical considerations are discussed.