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The Journal of Immunology, 1921, 6, 263 -270
Copyright © 1921 by The American Association of Immunologists, Inc.

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The Value of Tissue Extracts of Virus Pigs in the Production of Anti-Hog Cholera Serum

Thomas P. Haslam1

Abstract

I. Introduction. The experiments presented in this article were planned to ascertain whether expressed or extracted juices from the tissues of "virus pigs" could be used as a means of hyperimmunizing hogs in preparing anti-hog cholera serum by the Dorset-Niles method. Uhlenhuth (1) seems to have considered that the saline extract of the blood containing organs was about equal to the blood itself as a means of hyperimmunization. However his methods of hyperimmunization and of testing the potency of the serum vary so greatly from American methods that it is difficult to judge what might be expected under the conditions prevailing here. Our experiment was limited to muscle tissue because the juices recovered from the visceral organs contained many more bacteria than the juices recovered from the musculature.

II. Preparation of Muscle Virus. The pigs killed for virus in the routine production of anti-hog cholera serum were suspended head downward immediately after being killed.

Footnotes

1 This work was done while the author was with the Kansas Agricultural Experiment Station, Manhattan, Kansas.







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