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Antibody Studies

II. The Recovery of Antibody from Sensitized Antigens: Technic

From the Mulford Biological Laboratories

Abstract

The observations of many authors, as discussed in section 1, have demonstrated that a reversal of the antigen-antibody reaction is possible, at least in part, both in vivo and in vitro.

As has been stated in the preceding section, Kosakai (19) introduced the use of solutions of various sugars for the separation of hemolytic amboceptor from sensitized corpuscles and we first determined whether this technic could be applied to other antibodies. Since agglutinins are more easily handled than other antibodies and give fairly sharp-cut results, the preliminary work was done with these antibodies.

Separation of Agglutinins from Sensitized Bacteria.

In order that the steps in the process of dissociation may be followed and owing to the importance of seemingly slight variations in technic, it will be necessary to give the methods employed in detail, since it has been found that antibodies vary as to the ease with which they are dissociated and fall into classes in regard to the effect of different salts on such separation.