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Quantitative Studies of Complement Fixation

I. A Method¹

From the Departments of Medicine and Bacteriology, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital in the City of New York

Abstract

1. A simplified and improved quantitative method for the estimation of the fixation of C' is described. A single value of 1/n, 0.2, suffices for many variations in experimental conditions.

2. Adsorption of protein antigens by glass at the high dilutions used may be avoided by dilution with 0.1 per cent inactivated serum.

3. Data are given leading to the choice of 90 min as the most suitable fixation period at 37 C.

4. Comparisons are presented of the extent of C' fixation at 37 C and ice-box temperatures in the presence of varying amounts of C'.

Footnotes

¹ The work reported in this communication was carried out in part under the Harkness Research Fund of the Presbyterian Hospital and in part under a grant from the Rockefeller Foundation.

² Present address: Department of Bacteriology and Immunology, School of Public Health, Johns Hopkins University, Baltimore, Maryland.

³ On leave from the Bureau of Laboratories, New York City Department of Health.

⁴ John Simon Guggenheim Memorial Fellow.