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The Effect of Temperature and of Serum on the Detoxification of Staphylococcal Toxin by Interfacial Adsorption¹

From the Department of Biochemistry, Vanderbilt University, School of Medicine, Nashville, Tennessee and the Marine Biological Laboratory, Woods Hole, Massachusetts

Abstract

The behavior of staphylococcal toxin under the influence of interfacial adsorption, when it is emulsified at room temperature with a gas or with a relatively inert organic liquid, was described in a previous publication (1).

The object of the present investigation was to determine the effect of temperature and of serum protein on the detoxification of this toxin² by interfacial adsorption at a solution-air interface and at the interface which is formed when the toxin is emulsified with chloroform. The technic which was previously employed was modified by observing a strict temperature control during the process of emulsification and by the addition of rabbit serum prior to this treatment. The different samples of toxin were kept in an emulsified state by continuous shaking in a shaking machine for 24 hours at each of the following temperatures: 2, 14, 20, 25, and 35 C.

Footnotes

¹ The writer wishes to acknowledge his indebtedness to the donors of the Walter C. Hadley Fund for the financial aid given these experiments.

² Furnished through the courtesy of the Lederle Laboratories.