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A Study of the Reliability of Complement Fixation As a Method of Measuring the Activities of Sera of High, Medium, and Low Antibody Titer

From the Division of Laboratories and Research, New York State Department of Health, Albany

Abstract

The relationships between complement-fixing capacity, combining activity with homologous type-specific carbohydrate, and precipitative antibody-nitrogen content have been studied for antipneumococcus rabbit sera of high, medium, and low titer. Methods of estimating antibody-nitrogen values from complement-fixation titers have been developed in which these numerical relationships are applied.

As a means of defining the complement-fixing capacity of a standard quantity of antipneumococcus rabbit serum, with antigen, the expression
, which represents the total fixation corrected for change in the hemolytic activity of complement during incubation, was judged on the basis of its closer relationship to W_0.05, the maximally reactive dose of antigen, to be more accurate than either [(S + A)' - 1], the total fixation stated in "arbitrary" units, or
, the total fixation corrected for differences in the anticomplementary activity of 0.05 ml of serum.