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The Immunochemistry of Toxins and Toxoids

II. The Preparation and Immunologic Evaluation of Purified Tetanal Toxoid^*

From the Institute of Pathology, Western Reserve University, Cleveland 6, Ohio

Abstract

Methods employing methanol under controlled conditions of pH, ionic strength and temperature are described for the purification of tetanal toxoid. The antigenicity and the flocculative power of the purified material are quantitatively recovered and are concentrated about 300 times on a nitrogen basis over the parent toxoids.

The purified toxoids do not shock guinea-pigs sensitized to culture medium, indicating that they are relatively free of anaphylactogenic properties.

Procedures are given for the processing of the purified material to a stable state suitable for assay and immunization. Freezing and drying, sterile filtration in the presence of glycine and alum-precipitation do not alter the antigenic values of the purified tetanal toxoids.

The considerations discussed in the text suggest that the purified toxoids may prove useful for immunization of man against tetanus.

Footnotes

^* Aided by a grant from Wyeth Incorporated.

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