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The Journal of Immunology, 1946, 53: 259-268.
Copyright © 1946 by The American Association of Immunologists, Inc.
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Studies on Lymphogranuloma Venereum Complement-Fixing Antigens

I. Enhancement by Phenol or Boiling

Clara Nigg, Maurice R. Hilleman and Betty M. Bowser

Virus Laboratories, E. R. Squibb and Sons, New Brunswick, New Jersey

Abstract

1. The titer of lymphogranuloma venereum complement-fixing yolk sac antigens could be enhanced from 1–200 to 1–3200 by treatment with phenol under certain conditions of autolysis, temperature and time.
2. The titer could be similarly enhanced by boiling or autoclaving with or without phenol.
3. The properties of the antigen and the mechanism of enhancement are discussed.






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