HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kent, J. F. Articles by Goodman, R. Search for Related Content PubMed Articles by Kent, J. F. Articles by Goodman, R.

Studies in Complement Fixation

I. Spectrophotometric Titration of Complement; Construction of Graphs for Direct Determination of the 50 Per cent Hemolytic Unit

From the Division of Serology, Army Medical School, Army Medical Center, Washington, D. C.

Abstract

1. The use of a spectrophotometer in determining the percentages of hemolysis obtained in the titration of complement is described.

2. The mathematical treatment of the curve of hemolysis is reviewed, and a method described for the preparation of graphs with the aid of which the 50 per cent hemolytic unit of complement may be determined directly from the percentages of hemolysis observed in a given titration without constructing the curve of hemolysis.

3. The accuracy of such determinations of the 50 per cent unit was calculated for 13 titrations by reference to the standard unit determined by the method of least squares. The mean percentage of error was found to be +0.018, with a standard deviation of 0.29.

Footnotes

¹ Captain, Sanitary Corps, Army of the United States.

² Captain, Medical Corps, Army of the United States.

³ Lt. Colonel, Medical Corps, Army of the United States.

This article has been cited by other articles:

				R. P. Orange, M. D. Valentine, and K. F. Austen Release of Slow-Reacting Substance of Anaphylaxis in the Rat: Polymorphonuclear Leukocyte Science, July 21, 1967; 157(3786): 318 - 319. [Abstract] [PDF]

				J. F. KENT A Quantitative Study of the Complement-Hemolysin Relation Science, March 21, 1947; 105(2725): 316 - 317. [PDF]