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Comparative Sensitivity of the Extraneural and Intracerebral Neutralization Tests in Following the Antibody Response in Man to Vaccination with Western Equine Encephalomyelitis Virus¹

From the Laboratory of the Serviço de Estudos e Pesquisas Sôbre a Febre Amarela, Rio de Janeiro, Brazil

Abstract

The antibody response induced in man by vaccination with formolized Western equine encephalomyelitis virus was studied by means of extraneural and intracerebral neutralization tests.

The usual intracerebral test was found to be highly unsatisfactory for this purpose due to its inability to detect small amounts of antibody, a factor which precludes accurate measurement of the humoral immune response. As a consequence of this lack of sensitivity, the results by this test were such that the only interpretation possible was that vaccination elicited either no neutralizing antibody response or a minimal one.

By means of the extraneural test, however, it was possible to show that antibodies appeared as early as 2 weeks after vaccination, attained their maximal titer by 1 to 2 months, and then declined, to reach a low level by 9 or 10 months.

Footnotes

¹ The work on which these observations are based was conducted with the support and under the auspices of the Serviço de Estudos e Pesquises Sôbre a Febre Amarela, which is maintained jointly by the Ministry of Education and Health of Brazil and the International Health Division of The Rockefeller Foundation.

² Present address: California State Department of Public Health, Virus Laboratory, Berkeley.

³ Present address: Division of Virus and Rickettsial Research, Lederle Laboratories, Pearl River, New York.