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The Journal of Immunology, 1946, 52: 259-266.
Copyright © 1946 by The American Association of Immunologists, Inc.

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The Immunochemistry of Allergens1

VIII. PRECIPITIN FORMATION AND PASSIVE TRANSFER REACTIONS WITH ALLERGENIC PROTEINS FROM COTTONSEED AND CASTOR BEANS,2

E. J. Coulson, Joseph R. Spies, E. F. Jansen3 and Henry Stevens

Abstract

The allergenic proteic-polysaccharidic fractions, CS-1A from cottonseed and CB-1A from castor beans, were precipitinogenic when administered to rabbits. The allergenic fractions were less uniformly effective than ovalbumin in stimulating precipitin-formation.

The passive-transfer technic provided a more sensitive method for detecting antibodies in sera than the precipitin-method.

The passive-transfer sensitizing capacity of antisera, preserved with 0.25 per cent phenol and stored at 6 C, remained essentially unchanged for nearly five years.

Footnotes

1 Contribution from Allergen Investigations, Bureau of Agricultural and Industrial Chemistry, Agricultural Research Administration, U. S. Department of Agriculture.

2 For Paper VII of this series see Coulson, Spies, and Stovens, J. Allergy 1945, 16, 176–183.

3 Now with Western Regional Research Laboratory, Albany, California.







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