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The Immunochemistry of Allergens

V. Comparison of the Rates of Dialysis of Crystalline Ovalbumin and of the Cottonseed Allergen, CS-1A^1,2,

Abstract

The comparative rates of dialysis of crystalline ovalbumin and of the allergenic protein-carbohydrate fraction, CS-1A, from cottonseed were determined. CS-1A was detectable, by gross anaphylaxis, in the dialysates from mixtures of ovalbumin and CS-1A, and from mixtures of ovalbumin and cottonseed extracts, or from a solution of CS-1A alone, 30 minutes after the beginning of dialysis. Ovalbumin could not be detected in dialysates from these mixtures or from a solution of ovalbumin alone until dialysis had proceeded for a week or more. CS-1A was, therefore, shown to contain a readily diffusible antigen.

The predominant antigen appearing in the dialysate from unfractionated aqueous extract of cottonseed is closely similar to or identical with the allergenic proteic fraction, CS-51R.

Footnotes

¹ Contribution from Allergen Investigations, Bureau of Agricultural and Industrial Chemistry, Agricultural Research Administration, U.S. Department of Agriculture.

² For paper IV of this series see Coulson and Spies, This Journal, 46, 377–389.