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The Mouse-Protective Test as a Uniform Method of Assay for Antibacterial and Antiviral Sera

From Lederle Laboratories, Inc., Pearl River, N. Y.

Abstract

The mouse-protective technic described by Felton for evaluation of antipneumococcal sera has been used in determining potencies of antisera prepared by immunizing animals with Pasteurella multocida, Erysipelothrix rhusiopathiae, Streptococcus agalactiae (O90S), Clostridium chauvei; and the eastern and western types of the virus of equine encephalomyelitis. The results of the tests have revealed consistent differences in the length of time required for fatal consequences when similar lethal quantities of the different species of infecting agent were used. Nevertheless the protective endpoints were as clearly discernible in antisera specific for one organism as in antisera specific for another, and the mice receiving non-protective doses of serum died as regularly from infection with organisms requiring five days to kill as from infection with organisms requiring only one day. Observation-periods of 5 days for Cl. chauvei, P. multocida, types 1 and 2 and S. agalactiae, of 7 days for E. rhusiopathiae and the virus of eastern encephalomyelitis and of 9 days for the virus of western encephalomyelitis were considered to be satisfactory.