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The Specificity of Antibodies to Antigens Containing Two Different Determinant Groups

From the Institute of Biological and Medical Chemistry, University of Istanbul

Abstract

1. Immunization of rabbits with complex antigens containing simultaneously p-azo-phenyl-arsonic and m-azo-phenyl-sulfonic groups furnishes immune sera whose antibodies are precipitated quantitatively by subsequent addition of arsanil-azo-globulin and metanil-azo-globulin. No precipitate was produced by further addition of the homologous arsanil-metanil-azo-globulin.

2. Immunization of rabbits with complex antigens containing simultaneously azo-phenyl-arsonic groups and di-iodo-tyrosin groups gives rise to the production of immune sera whose antibodies are precipitated quantitatively by the subsequent addition of arsanil-azo-protein and iodo-protein. No further precipitate is produced by the addition of the complex arsanil-azo-iodo-protein. An immune serum with the same properties is produced by the injection of a mixture of arsanil-azo-globulin and iodo-globulin into rabbits.

3. Our experiments demonstrate that immunization with a complex antigen AB containing two kinds of determinant groups A and B leads to the formation of antibodies of the type anti-A and anti-B. No indication for the presence of anti-A-B was found. This supports the assumption that antibodies are of a single specificity.