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The Immunochemistry of Allergens

III. Anaphylactogenic Potency of the Electrophoretic Fractionation Products of CS-1A from Cottonseed^1,2,

Abstract

Data collected in estimating the anaphylactic sensitizing and shocking capacity of cottonseed allergenic fractions and crystalline ovalbumin were used to construct dosage-response curves according to the system of computation developed by Bliss. Values for median lethal doses (LD 50) and median sensitizing doses (SD 50) were computed. On the basis of nitrogen content the shocking capacity for electrophoretic fractionation products of CS-1A and for ovalbumin were all of the same order. However, large differences were found in their sensitizing potency. Thus the sensitizing capacity of the high carbohydratecontaining fraction, CS-56R, was eight-fold greater than that of the low-carbohydrate fraction, CS-51R. Inasmuch as these two fractions have the same antigenic specificity, the greater sensitizing power of CS-56R was attributed to its larger molecular structure resulting from the high proportion of combined polysaccharide. The sensitizing capacity of ovalbumin was ten-fold greater than CS-56R.

Footnotes

¹ Contribution from Allergen Investigations, Bureau of Agricultural and Industrial Chemistry, Agricultural Research Administration, U. S. Department of Agriculture.

² For paper II of this series see Coulson, Spies, and Stevens, This Journal, 1943, 46: 347–365.