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The Journal of Immunology, 1943, 46: 177-182.
Copyright © 1943 by The American Association of Immunologists, Inc.

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A Comparison of the Collodion-Particle Technic with Other Methods of Measuring Antibody

Francis C. Lowell

From the Evans Memorial, Massachusetts Memorial Hospitals, and the Department of Medicine, Boston University School of Medicine, Boston, Massachusetts

Abstract

The collodion-particle technic described by Cannon and Marshall was compared with the gelatin-interface test and the complement-fixation test. The comparisons were made using rabbit antisera for ovalbumin, ragweed-pollen extract and horse-serum. The collodion-particle technic consistently permitted the greatest dilution of serum without loss of the ability to give an easily visible antigen-antibody reaction. The titers obtained with the collodion-particle technic paralleled those obtained with the gelatin-interface and the complement-fixation tests.







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