HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gallagher, F. W. Articles by Jones, L. R. Search for Related Content PubMed Articles by Gallagher, F. W. Articles by Jones, L. R.

Preparation and Use of Rh Testing Sera

From the Department of Bacteriology, St. Louis University School of Medicine, St. Louis, Missouri

Abstract

The existence of an antigen in the erythrocytes of rhesus monkeys, and accordingly designated as Rh, which is found also in the red blood cells of most human beings, was reported in 1940 by Landsteiner and Wiener (1). Methods for preparing anti-Rh sera in experimental animals and of testing for the antigen in erythrocytes are described by these same workers (2). It has been shown, also, that Rh agglutinins may appear in the blood serum of certain individuals who are devoid of the antigen, and that this appearance may be associated with disorders of clinical importance, e.g., transfusion reactions and erythroblastosis fetalis (3, 4). In connection with our study (5) of these problems we have employed antisera from animal and human sources in testing for the Rh antigen. In the present communication are reported our experiences in the production of animal sera and in the absorption of these sera for the purpose of enhancing their usefulness.