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Proteic Fractions of a Strain of Group A Hemolytic Streptococcus

IV. Azo Dyes Containing Hemolytic Streptococcal Protein¹

From the Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York City

Abstract

1. By combination of hemolytic streptococcal protein with an azo component and study of the chemical and serological properties of the resulting red azo antigen it is shown that only a portion of the azo-protein reacts serologically.

2. An antiserum to the dye-streptococcal protein contained anti-hapten since it precipitated its red analogs, R-salt-azobiphenylazo egg albumin and R-salt-azo-biphenylazo-horse serum albumin.

Footnotes

¹ The work reported in this communication was carried out under the Harkness Research Fund of the Presbyterian Hospital.

² Fellow of the Norwegian Malthe and Pasteur Foundations, 1939–40; Commonwealth Fund Fellow, 1940–1.