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Studies with Colored Antigens¹

I. The Rate of Disappearance of Dye-Albumin from the Blood

From the Departments of Physiology and Pediatrics, Harvard Medical School, from the Department of Physiology, College of Physicians and Surgeons, Columbia University, and from the Infants' and the Children's Hospitals, Boston

Abstract

By means of conjugating R-salt, a dye, with recrystallized egg-albumin through tetrazotized benzidine, the specificity of the original albumin was altered. This conjugated dye-protein was then injected intravenously into rabbits and a dog and the rate of its disappearance from the blood-plasma was determined by analyzing subsequent samples of plasma quantitatively for the presence of dye by means of spectrophotometric determinations. The dye-protein was found to disappear rapidly from the plasma, 30 to 80 per cent (depending upon amount injected) escaping in the first hour, complete removal being effected in 12 to 24 hours.

Only a small fraction (5 per cent or less) of the dye-protein was taken up by the cellular elements of the blood in vitro, and none could be detected in the urine by colorimetric or immunological methods. The rapid escape of this substance from the plasma cannot, therefore, be explained either by a reaction between it and the cellular elements of the blood or by excretion through the kidney.

Footnotes

¹ This study was supported in part by a grant from the Leal Fund for the Study of Asthma.