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Influence of Electrolytes on the Cutaneous Reactivity of Seitz-Filtered Ragweed Solutions¹

From the Allergy Clinic, Cincinnati General Hospital, and the Biochemistry Laboratory, University of Cincinnati

Abstract

1. Methods for the preparation of "electrolyte-free" ragweed pollen extracts (i.e. having an electrical conductivity less than 20 x 10^–6 reciprocal ohms) are presented.

2. The reduction of the electrolyte-content of a ragweed pollen extract per se did not significantly diminish its capacity to produce intracutaneous reactions in ragweed pollen-sensitive subjects.

3. If "electrolyte-free" ragweed pollen extracts of specified concentrations were passed through Seitz filters, the filtrates did not produce intracutaneous reactions in ragweed pollen-sensitive subjects.

4. The specific activity of Seitz-filtered "electrolyte-free" ragweed pollen extracts could be restored or enhanced by the addition of electrolytes in proper concentration.

5. A definite relationship between the specific activity of Seitz-filtered ragweed pollen extracts and the electrolytes contained therein has been demonstrated. This relationship was found to be dependent upon: (a) a certain alteration of unknown character produced by filtration through a Seitz filter-pad. (b) the concentration and type of contained electrolytes.

Footnotes

¹ This investigation was aided by a grant from Miss Mary Hanna, Cincinnati, Ohio.