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The Proteins and Antibodies of Serum after Prolonged Dialysis¹

III. Serial Immunization with Quantitative Antibody Estimations

From the Department of Bacteriology and Experimental Pathology, School of Medicine, Stanford University, California

Abstract

When single samples of serum are subjected to prolonged dialysis by both the intermittent and continuous methods, the former is found to result in a greater precipitation of total protein, globulin, and antibody.

Sera obtained from a group of animals at successive points in the process of immunization with type 2 pneumococcus give evidence of changing protein- and antibody-relationships. The later sera show a progressively greater increment of water-soluble over total globulin, the presence of relatively larger proportions of total antibody in the water-soluble globulin and this antibody is probably the more multivalent. The evidence in its entirety is considered to support the thesis proposed in previous papers (1, 2); that the first, less avid antibody produced by the rabbit is associated with the water-insoluble globulin, and as immunization proceeds the increasing amounts of multivalent immune body are composed of water-soluble globulin. The specific globulin-association of the antibody produced by this species is thus primarily dependent on the stage during immunization at which serum is obtained.

Footnotes

¹ This work was supported in part by a grant from the Rockefeller Fluid Research Fund of the School of Medicine, Stanford University.