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The Proteins and Antibodies of Serum after Prolonged Dialysis¹

II. Dialysis Without Periodic Removal of Precipitate

From the Department of Bacteriology and Experimental Pathology, School of Medicine, Stanford University, California

Abstract

The fractionation of rabbit sera by prolonged dialysis against distilled water with removal of the precipitate at the termination of the period of dialysis has been investigated.

The character of the precipitate is comparable to that previously described for sera similarly dialyzed with successive removals of precipitate as it formed. The quantity and composition differ, however. In this case there is less total precipitate, primarily because of the sedimentation of less albumin-like protein, and partly also because less globulin precipitates.

The quantitative and qualitative distribution of antibody between the dialyzed fractions lends support to the view previously expressed that the specific globulin with which immune body is associated depends on the intensity of the immunizing process. In the rabbit, multivalent antibody appears to be associated with the water-soluble globulin. As this type of antibody increases in amount with progressive vaccination, an increasingly greater part of the total antibody of the serum is found in association with this globulin, while a progressively smaller part occurs in the water-insoluble portion.

Footnotes

¹ This work was supported in part by a grant from the Rockefeller Fluid Research Fund of the School of Medicine, Stanford University.