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The Proteins and Antibodies of Serum after Prolonged Dialysis¹

I. Periodic Removal of Precipitates

From the Department of Bacteriology and Experimental Pathology, School of Medicine, Stanford University, California

Abstract

The fractionation by prolonged dialysis of the proteins and antibodies of normal and immune rabbit sera has been investigated.

A characteristic serial precipitation occurs when precipitates are removed from the dialyzing bag at intervals. The precipitates change in character, quantity and composition as dialysis progresses. Half or more of the total precipitate is composed of an albumin-like protein.

The content of antibody in the serial precipitates roughly parallels their total-nitrogen values. The largest precipitates are also richest in antibody per unit of mass.

The antibody-activities displayed by the dialyzed fractions of two of three immune sera indicated that the better-quality antibody of a serum is associated with the water-soluble globulin. Since the "multivalence" of antibody generally increases with progressive immunization, the specific globulin-association of immune body in any particular serum may depend on the immunizing treatment.

Footnotes

¹ This work was supported in part by a grant from the Rockefeller Fluid Research Fund of the School of Medicine, Stanford University.