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Comparison of Various Physical Means of Liberation of the Agglutinogen from H. Pertussis in Phase I¹

Department of Bacteriology, University of Pennsylvania Medical School

Abstract

The sonic method of cellular disintegration is the most effective of the six physical means tried for liberation of the phase I-agglutinogen of H. pertussis. The low temperature ball-mill is next and offers more effective means of obtaining the agglutinogen than grinding the dried organisms at room temperature. Semi-quantitative agglutinin-absorption was used as the method of biological assay. Soluble nitrogen content of the extracts does not afford a satisfactory criterion of relative efficiency of extraction of surface-antigen.

Footnotes

¹ This is paper III in a series "Studies with H. Pertussis". This work has been aided by a grant from the United States Public Health Service.