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Studies on Antipneumococcic Rabbit Serum

II. The Relation Between Mouse-Protective Units and Precipitin-Nitrogen

Biological Laboratories of E. R. Squibb & Sons, New Brunswick, N. J.

Abstract

The ratios of mouse-protective units to antibody-nitrogen content of types I, II, III, IV, V, VII, and VIII antipneumococcic rabbit plasma, serum, and concentrates have been determined. By comparison with the official standard sera for types I, II, V, VII and VIII, and with tentative standards for types III and IV, the values obtained vary from about 900 units per milligram of antibody-nitrogen for type II and about 1100 for types I, IV, and VIII, to about 1500 for types III and V, and 2000 for type VII. It is felt that the greater part of the variation is due to the difficulty of determining the mouse-protective units for the various types and titrating the sera in terms of them.