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The Journal of Immunology, 1940, 39: 73-83.
Copyright © 1940 by The American Association of Immunologists, Inc.

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Antigen-Antibody Reactions in Paramecium: The Aurelia Group

Alan W. Bernheimer and James A. Harrison

Biology Department of Temple University and the Biological Institute, Philadelphia

Abstract

1. The presence of precipitin, complement-fixing and immobilizing antibodies in the serums of rabbits immunized with paramecia is demonstrated.
2. A standard technic for performing the immobilization-test is presented. This test is applied to the investigation of serologic specificity in Paramecium.
3. Paramecium multimicronucleatum may be immobilized by anti-multimicronucleatum serum, but P. aurelia and P. caudatum are not immobilized by anti-multimicronucleatum serum. Analogous specificity is exhibited by anti-caudatum serum and by anti-aurelia serum.
4. When a number of stocks of a single species of paramecium isolated from geographically remote localities are tested with the antiserum prepared with one of these stocks, one or more of the stocks is immobilized; others are not. This is true for each of the three species studied, and, therefore, for each there is evidence suggestive of the existence of two or more antigenic types.







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