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Neutralization of the Bacteriophage

Department of Bacteriology and Experimental Pathology, Stanford University, California

Abstract

1. The time required for an anticoliphage to lyse an actively growing culture of Esch. coli is proportional to the initial concentration of the phage. Other conditions being constant, the concentration of phage in different samples can be determined by noting the relative length of time required for the reduction of a constant initial concentration of bacteria to an arbitrary turbidity endpoint.

2. The time required for a given initial concentration of phage to reduce the concentration of bacteria to this arbitrary end-point is increased in the presence of an antilytic serum. Quantitative measurements, under the dynamic conditions of these experiments, indicate that the neutralization of phage over a wide range of concentrations of phage and of antiserum, goes almost to completion within a few minutes.

3. Observations are presented which suggest that the preliminary union between phage and antiphage is an adsorption process.