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A Ring Precipitin Test for Estimating the Concentration of Antibody in Small Amounts of Immune Serum

From the Department of Bacteriology, School of Medicine, The George Washington University, Washington, D. C.

Abstract

A ring precipitin method has been described for estimating the concentration of antibody in small amounts (0.03 to 0.21 cc. is sufficient for a preliminary titration and for duplicate checks) of immune serum. The test depends on allowing an optimal amount of antigen to diffuse into increasing dilutions of the antiserum in such a way that the smallest detectable concentration of antibody is precipitated in the form of a visible ring on the upper surface of the gelatinized antigen.

Data are presented on the control of variables and on the quantitative aspects of the procedure in the titration of anti-horse serum, as checked by the "optimal proportions" method of Dean and Webb. Preliminary information on the titration of other antigen-antibody systems has been given.