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Studies on the Antigenic Substances of Clostridium Parabotulinum. II

From the George Williams Hooper Foundation for Medical Research and Department of Bacteriology, University of California Medical School, San Francisco

Abstract

In studies on the complement fixation and precipitin reactions (1, 2, 3), it has been shown that extracts prepared by alternate freezing and thawing of suspensions of Clostridium parabotulinum are highly group specific when tested with antiserums produced by the inoculation of intact organisms. It was thought that it would be of interest to test the antigenic properties of such extracts when injected into the animal body.

Extracts were prepared from seven representative strains of Cl. parabotulinum by various methods. Twenty-four hour cultures in glucose veal broth were centrifugalized and washed three times in salt solution. In one series the organisms were suspended in neutral distilled water, in a second in neutral 0.85 per cent salt solution, and in a third in alkaline salt solution (pH 8.0). The suspensions were frozen in a mixture of crushed ice and salt and thawed rapidly by plunging into warm water (70°C.).