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Active Immunization of White Mice by a Non-Polysaccharide and Probably Non-Protein Derivative of the Pneumococcus¹

From the Department of Preventive Medicine and Hygiene, Harvard Medical School, Boston

Abstract

The inferences which may be drawn from this study are:

1. White mice may be immunized with a fraction of pneumococcus which is soluble in copper acetate in the presence of saturated sodium chloride and may be extracted with alcohol from the dried copper-free filtrate. This antigen gives a negative Molisch reaction and is probably free from protein. Also no precipitation occurs with antipneumococcus horse serum.

2. The soluble carbohydrate of Avery and Heidelberger in proper dosage prevents the immunizing effect of this antigen.

3. The immunizing antigen has been extracted by the copper method from samples of both type I and type II polysaccharide.

4. Demonstrable immunity is developed in seventy-two hours after intraperitoneal injection, and in a shorter time following intravenous injection.

5. Immunity after a single injection of antigen remains maximum for at least three weeks.

6. The immunity conferred on white mice from a single injection is type specific.

Footnotes

¹ Read before the American Association of Immunologists, Philadelphia, April 27, 1932.

This is one of a series of studies carried out in part under a grant from the Influenza Commission, and in part under a grant from the Littauer Pneumonia Fund.