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Studies on Crystalline Urease

II. Hemagglutinating Properties of Urease

(From the Department of Bacteriology and the Department of Physiological Chemistry, New York Homeopathic Medical College and Flower Hospital, New York, N. Y.)

Abstract

Crystalline urease in solution produced agglutination of the erythrocytes of the rabbit and of the mouse, but not of the sheep.

In the tests where high concentrations of urease were used hemolysis occurred, complicating the results. This was subsequently controlled by the use of a buffer solution, when agglutination could be readily seen.

After agglutination the urease was found to be active.

Agglutination could not be demonstrated in the whole blood of rabbits dead after urease injection.

Crystalline urease represents a highly purified form of toxic hemagglutinin (10).