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The Variables in the Mouse¹

Protection Test of Antipneumococcus Serum

From the Department of Preventive Medicine and Hygiene, Harvard Medical School, Boston

Abstract

There are two methods in general use for the estimation of the protective value of antipneumococcus serum. The first consists in measuring the protective value of a unit amount of serum by varying the dose of the culture; in the second method, conversely, a constant dose of organism is employed against varying dilutions of the serum. The first method was originated by Neufeld (1), who also tried the second, and found that with the very dilute cultures he employed his method was superior to the other which was suggested by Landmann (2).

In the first method, as described by Neufeld, 0.2 cc. of antipneumococcus serum was injected, either subcutaneously or intraperitoneally, three hours before the injection of virulent pneumococci in the following series of doses: 0.2, 0.1, 0.01, 0.001, 0.0001 cc. The culture he used was of such virulence that control mice injected in duplicate with 0.000001 cc. died in forty-eight hours.

Footnotes

¹ This is one of a series of studies carried out in part under a grant by the Influenza Commission of the Metropolitan Life Insurance Company.