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The Relation of the Bacterial Precipitin Reaction to the Ramon Flocculation Phenomenon

From the Department of Bacteriology, College of Physicians and Surgeons, Columbia University, New York City.

Abstract

1. Flocculation in mixtures of filtrate of C. diphtheriae and antitoxin is not limited by agglutination types (2,11) of the organisms which serve for the toxin production. Precipitation in mixtures of toxic filtrate and agglutinating serum is restricted to agglutination types.

2. Removal of the non-toxic precipitinogens from the toxic filtrate after contact with a specific precipitating serum has little, if any, effect upon the Lf of the toxin; but prevents subsequent precipitation of the filtrate with the homologous precipitating serum.

3. The property in the toxic filtrate essential for flocculation with antitoxin is destroyed at a temperature of 60°C. for one-half hour whereas, the property essential for precipitation of agglutinating serum is not destroyed at a temperature of 85°C. for one hour.

4. Removal of the bacterial antibody from antitoxic serum by absorption of the serum with bacterial extracts does not prevent subsequent flocculation of the serum with the toxic filtrate, whereas, the agglutinating serum treated in the same manner no longer precipitates with the toxic filtrate.

5. The Ramon reaction is probably due to interaction between toxin and antitoxin and not between bacterial precipitinogen and precipitin.