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Biochemical Studies of Bacterial Derivatives

XI. Skin Reactions in Man with Human Tubercle Bacillus Protein MA-100—preliminary Report¹

Philadelphia, Pennsylvania

Abstract

Human tubercle bacillus protein MA-100 was isolated by Masucci and McAlpine (1) in November, 1929, from the filtrate of cultures of the human tubercle bacillus H-37, grown on Long's synthetic culture medium. This medium contains no protein, but contains asparagin as a source of organic nitrogen. All proteins present with various other substances, not medium constituents, therefore, in the filtrate must have been synthesized by the tubercle bacillus.

One of the most important of these substances is a specific polysaccharide, closely associated with the proteins. However, Masucci and McAlpine have been able to isolate not only a protein MA-100 free from the polysaccharide, but to obtain the poly-saccharide free from the protein. The designation MA-100 is given the protein to classify the material with the chart published by Johnson (2).

Only one phase of these investigations; namely, the skin sensitivity of man to this particular human tubercle bacillus protein MA-100, when the protein is introduced intradermally, is dealt with in this communication.

Footnotes

¹ From the Medical Service of the Jefferson and Pennsylvania Hospitals, Philadelphia, and the White Haven Sanatorium. This study is a part of the investigation outlined by the Research Committee of the National Tuberculosis Association to obtain knowledge of the chemical constituents of the tubercle bacillus and of their biological activity (3). The human tubercle bacillus protein MA-100 was supplied by the Mulford Laboratories, Glenolden, Pa.