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Use of Broth Culture Filtrates in Anaphylaxis Experiments

From the Department of Bacteriology and Immunology, Vanderbilt University Medical School, Nashville, Tennessee

Abstract

Experiments were made on two questions. First, the primarily toxic (anaphylactoid) action was studied by intravenous injection of normal guinea pigs with formolized and unformolized filtrates of diphtheria and other bacilli and with the non-bacterial constituents of the filtrates (uninoculated broth, solutions of phenol and of formol). The possibility of sensitization to the non-bacterial constituents during immunization with the filtrates was studied by intravenous tests on animals previously given repeated subcutaneous injections of formol solution or of formolized broth. With diphtheria filtrates, neither the primary toxicity nor the possibility of sensitization to the broth constituents, introduced significant complications in their use as material for active sensitization and for subsequent intravenous tests. The adaptability of broth culture filtrates of other bacteria to anaphylaxis experiments would depend upon the quantitative effectiveness of the antigens they liberate into their culture fluids.