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This Article Full Text Full Text (PDF) All Versions of this Article: jimmunol.0900332v1 183/5/3033    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Google Scholar Articles by Kim, S. Articles by Min, B. PubMed PubMed Citation Articles by Kim, S. Articles by Min, B.

Basophils Can Directly Present or Cross-Present Antigen to CD8 Lymphocytes and Alter CD8 T Cell Differentiation into IL-10-Producing Phenotypes¹

Department of Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195

There is increasing evidence suggesting that basophils play a critical role in developing Th2-type immunity both in vitro and in vivo. We previously reported that basophils cocultured with naive CD4 T cells stimulated with Ag promote the differentiation of the T cells into IL-4-producing Th2 cells. In the present study, we examined the roles of basophils during CD8 T cell activation. Although stimulating OVA-specific OT-I CD8 T cells with OVA peptide-pulsed splenic dendritic cells primarily induced the production of IFN-, adding basophils into the coculture induced IL-10 production. Surprisingly, basophils were capable of directly presenting peptide Ag or of cross-presenting protein Ag to CD8 T cells. CD28-mediated costimulation dramatically enhanced T cell IL-10 production, yet neither ICOS nor CD86 was involved in IL-10 production. Basophil-mediated IL-10 induction was greatly diminished without IL-4 or IL-6, indicating that these cytokines are necessary for programming CD8 T cell IL-10 production. Adding IL-4 or IL-6 into CD8/APC coculture was not sufficient to induce IL-10 production; however, the presence of both cytokines significantly induced IL-10 production without basophils. Finally, CD8 T cells producing IL-10 induced by basophils did not display regulatory cell functions. Collectively, these results suggest a novel function of basophils that act as professional APCs to present Ag to CD8 T cells, thus inducing IL-10 production.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This study was supported by the start-up fund from the Cleveland Clinic Foundation (to B.M.).

² Current address: Biosafety Level 3 Laboratory of Peking University Health Science Center, Beijing, China.

³ Address correspondence and reprint requests to Dr. Booki Min, Department of Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195. E-mail address: minb{at}ccf.org

⁴ Abbreviations used in this paper: TSLP, thymic stromal lymphopoietin; MHC I, MHC class I; MHC II, MHC class II; PFA, paraformaldehyde; DC, dendritic cell.