HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Data Supplement Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wang, S. Articles by Wang, J.-X. Search for Related Content PubMed PubMed Citation Articles by Wang, S. Articles by Wang, J.-X.

TRBP Homolog Interacts with Eukaryotic Initiation Factor 6 (eIF6) in Fenneropenaeus chinensis¹

School of Life Sciences, Shandong University, and The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education, Jinan, Shandong, China

The HIV transactivating response RNA-binding protein (TRBP) plays an important role in many biological processes. We have cloned three cDNAs from newly identified genes in the TRBP family from Fenneropenaeus chinensis. These genes have been designated Fc-TRBP1–3. Recombinant Fc-TRBP1, which was produced in Escherichia coli, was used for panning of a T7 phage display library of the Chinese shrimp hemocytes. From this panning, Fc-eukaryotic initiation factor 6 (Fc-eIF6) was isolated and sequenced. Fc-eIF6 was then cloned, recombinantly expressed, and shown to interact with Fc-TRBP by the performance of pull-down assays and Far Western blot analysis. Expression of Fc-TRBP was detected in many tissues, with elevated expression in the heart, gill, and intestine in the early stages of infection by the white spot syndrome virus (WSSV), and enhanced expression in most tissues following challenge with Vibrio anguillarum. Western blot studies confirmed the increased expression of Fc-TRBP in the gill after WSSV infection. The expression pattern of eIF6 was also analyzed and its expression was also up-regulated in intestine of WSSV-challenged shrimp. The replication of WSSV was reduced after injection of Fc-TRBP. These results indicate that Fc-TRBP and Fc-eIF6 may be components of the RNA-induced silencing complex (RISC), and thereby play a crucial role in the antiviral defense response of shrimp.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by grants from the Natural National Science Foundation of China (no. 30770282), the Major State Basic Research Development Program of China (973 program, no. 2006CB101806), the National High Technology Research and Development Program of China (863 Program, no. 2007AA09Z425), and the Ph.D. Program Foundation of the Ministry of Education of China (no. 20060422034).

² Address correspondence and reprint requests to Dr. Jin-Xing Wang, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China. E-mail address: jxwang{at}sdu.edu.cn

³ Abbreviations used in this paper: TRBP, HIV transactivating response RNA-binding protein; Ago2, Argonaute 2; Dig, digoxigenin; DSRM, dsRNA-binding motif; eIF6, eukaryotic initiation factor 6; 4-CIN, 4-chloro-1-naphthol; IPTG, isopropyl β-D-thiogalactoside; miRNA, microRNA; NC, nitrocellulose; ORF, open reading frame; PKR, protein kinase R; RISC, RNA-induced silencing complex; RNAi, RNA interference; TAR, trans-activation response; UTR, untranslated region; WSSV, white spot syndrome virus.

⁴ The online version of this article contains supplemental material.