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NK Cell Enhancement of Antigen Presentation by B Lymphocytes¹

Paula Jennings^*, and Dorothy Yuan^2,

^* Graduate Program in Immunology and Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390

Ag presentation to CD4 T cells can be mediated by a number of cell types depending on the anatomical site in which Ag is first encountered. For blood borne Ags, cells localized in situ in the spleen should be major players. There is now much evidence that B cell Ag presentation may be particularly important in the priming of memory T cells. The majority of NK cells are also localized the spleen. Inasmuch as we have previously shown that NK cells can modulate various aspects of B cell differentiation, we entertained the possibility that NK cells can also influence Ag presentation by B cells. By specific depletion of NK cells before immunization, we show herein that NK cells play an important role in modulating the ability of B cells to process and present Ag to T cells. These effects are particularly important in the generation of memory T cells. The findings are further substantiated by in vitro experiments showing that the enhancement does not require IFN- but is mediated by direct cell-cell interaction. These results show, for the first time, that the rapid activation of a component of the innate response can even exert effects on the Ag-specific memory response.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ The study was supported by The National Institutes of Health Grant R01: AI69253.

² Address correspondence and reprint requests to: Dr. Dorothy Yuan, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390. E-mail address: dorothy.yuan{at}utsouthwestern.edu

³ Abbreviations used in this paper: DC, dendritic cell; TD, T cell dependent; MHC II, MHC class II; MLN, mesenteric lymph node; MNC, mononuclear cell; FL, fluorescein; DC, dendritic cell; WT, wild type; KLH, keyhole limpet hemocyanin; SA, streptavidin.