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Chemotaxis and Phagocytosis in Neutrophils Is Independent of Coronin 1¹

Biozentrum, University of Basel, Basel, Switzerland

The WD repeat protein family member coronin 1 is exclusively expressed in leukocytes, where it colocalizes with the cortical cytoskeleton. Although initially coronin 1 was believed to regulate F-actin dynamics such as leukocyte motility, phagocytosis, and membrane ruffling, recent work showed that in macrophages, T cells, and B cells, coronin 1 is dispensable for these F-actin dependent processes, instead being involved in the regulation of calcium dependent signaling reactions. In this study, we show that in mice lacking coronin 1 neutrophil populations developed normally, and that coronin 1-deficient neutrophils are fully functional with respect to adherence, membrane dynamics, migration, phagocytosis and the oxidative burst. Therefore, these data argue against a role for coronin 1 in the modulation of F-actin and suggest that coronin 1 is dispensable for neutrophil functioning.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by grants from the Swiss National Science Foundation, the Olga Mayenfisch Stiftung, the Swiss Life Jubilaeumsstiftung, and the Canton of Basel-Stadt.

² Address correspondence and reprint requests to Dr. Jean Pieters, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, Switzerland. E-mail address: jean.pieters{at}unibas.ch

³ Abbreviations used in this paper: WT, wild type; HBSS, Hanks’ Balanced Salt Solution; MFI, mean fluorescence intensity.