HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Data Supplement Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jacobelli, J. Articles by Krummel, M. F. Search for Related Content PubMed PubMed Citation Articles by Jacobelli, J. Articles by Krummel, M. F.

Myosin-IIA and ICAM-1 Regulate the Interchange between Two Distinct Modes of T Cell Migration¹

Department of Pathology, University of California San Francisco, San Francisco CA 94143

How T cells achieve rapid chemotactic motility under certain circumstances and efficient cell surface surveillance in others is not fully understood. We show that T lymphocytes are motile in two distinct modes: a fast "amoeboid-like" mode, which uses sequential discontinuous contacts to the substrate; and a slower mode using a single continuously translating adhesion, similar to mesenchymal motility. Myosin-IIA is necessary for fast amoeboid motility, and our data suggests that this occurs via cyclical rear-mediated compressions that eliminate existing adhesions while licensing subsequent ones at the front of the cell. Regulation of Myosin-IIA function in T cells is thus a key mechanism to regulate surface contact area and crawling velocity within different environments. This can provide T lymphocytes with motile and adhesive properties that are uniquely suited toward alternative requirements for immune surveillance and response.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by National Institutes of Health (R01AI052116) and the Leukemia and Lymphoma Society (to M.F.K.).

² Address correspondence and reprint requests to Dr. Matthew F. Krummel, Department of Pathology, University of California San Francisco, 513 Parnassus Avenue, San Francisco CA 94143. E-mail address: matthew.krummel{at}ucsf.edu

³ Abbreviations used in this paper: shRNA, short hairpin RNA; TIRF, total internal reflection fluorescence; CMTMR, (5(and-6)-(((4-chloro-methyl)benzoyl)amino)tetramethylrhodamine.

⁴ The online version of this article contains supplemental material.