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* Institute for Animal Physiology, University of Munich, Munich, Germany; and
Animal Breeding and Genomics Centre, Wageningen University, Wageningen, The Netherlands
FcRs have multifaceted roles in the immune system. Chicken FcRs were demonstrated on macrophages decades ago; however, only recently the chicken Ig-like receptor AB1, encoded in the leukocyte receptor complex, was molecularly identified as a high-affinity FcR. The present study was initiated to identify additional receptors with the capability to bind chicken immunoglobulins. Based on database searches, we cloned a novel chicken FcR, designated gallus gallus FcR (ggFcR), which was shown to bind selectively chicken IgY. The receptor consists of four extracellular C2-set Ig domains, followed by a transmembrane region containing arginine as a positively charged amino acid and a short cytoplasmic tail. ggFcR associates with the common 
-chain, indicative for an activating receptor, and real-time RT-PCR revealed high expression on PBMC, thrombocytes, and macrophages. The genomic organization is similar to most Ig-like receptor genes, where each Ig domain is encoded by a separate exon. Additionally, the ggFcR signal peptide is encoded by two exons, the second of which is 36 bp, a hallmark for genes encoded in the leukocyte receptor complex. Phylogenetic analysis also showed a relationship to genes encoded in the leukocyte receptor complex. Surprisingly, ggFcR is not encoded in the leukocyte receptor complex, but it is located as a single isolated gene at the extremity of chicken chromosome 20.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by Grants VI459/2-1 and GO489/3-6 from the Deutsche Forschungsgemeinschaft.
2 Address correspondence and reprint requests to Dr. Birgit Viertlböck, Institute for Animal Physiology, Veterinärstrasse 13, 80539 München, Germany. E-mail address: Birgit.Viertlboeck{at}lmu.de
3 Abbreviations used in this paper: LRC, leukocyte receptor complex; BLAST, basic local alignment search tool; CHIR, chicken Ig-like receptor; EST, expressed sequence tag; FCRL, FcR-like molecules; gg, gallus gallus; KIR, killer cell Ig-like receptor; LILR, leukocyte Ig-like receptor; L3MBTL, lethal(3)malignant brain tumor-like protein; RH, radiation hybrid.
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