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Cutting Edge: An IL-17F-Cre^EYFP Reporter Mouse Allows Fate Mapping of Th17 Cells¹

First Medical Department, Johannes Gutenberg University of Mainz, Mainz, Germany

The need for reporter lines able to faithfully track Th17 cells in vivo has become an issue of exceptional importance. To address this, we generated a mouse strain in which Cre recombinase is expressed from the IL-17F promoter. Crossing the IL-17F-Cre allele to a conditional enhanced yellow fluorescent protein (EYFP) reporter mouse yielded the IL-17F-Cre^EYFP strain, in which IL-17F expression is twinned with EYFP in live IL-17F-expressing cells. Although we demonstrate that IL-17F expression is restricted to CD4⁺ T cells during experimental autoimmune encephalomyelitis, IL-17F-Cre^EYFP CD8 T cells robustly expressed IL-17F in response to TGF-β, IL-6, and IL-23. Fate mapping of IL-17F-expressing reporter T cells revealed a significant down-regulation of Th17 cytokines after homeostatic expansion in RAG1-deficient animals. Despite this loss of effector phenotype, committed Th17 cells were resistant to Foxp3 expression in vitro or in vivo. Thus, the IL-17F-Cre strain furthers our understanding of Th17 biology.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was funded by the FP6 Marie Curie Research Training Network MRTN-CT-2004-005632 (IMDEMI), Deutsche Forschungsgemeinschaft Grants SFB 490 and SFB/TR 52, and funds from the Boehringer Ingelheim Stiftung (to A.W.).

² Address correspondence and reprint requests to Dr. Ari Waisman, First Medical Department, University of Mainz, Obere-Zahlbacherstrasse 63, 55131 Mainz, Germany. E-mail address: waisman{at}uni-mainz.de

³ Abbreviations used in this paper: EAE, experimental autoimmune encephalomyelitis; BAC, bacterial artificial chromosome; EYFP, enhanced yellow fluorescent protein; MOG, myelin oligodendrocyte glycoprotein; Tc, cytotoxic T cell; Treg, T regulatory cell; iTreg induced Treg.

⁴ The online version of this article contains supplemental material.

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