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Transfusion of IgG-Opsonized Foreign Red Blood Cells Mediates Reduction of Antigen-Specific B Cell Priming in a Murine Model¹

Davor Brinc^*,, Hoang Le-Tien^*,, Andrew R. Crow^*,, Vinayakumar Siragam^*,, John Freedman^, and Alan H. Lazarus^2,*,,

^* Canadian Blood Services, Department of Laboratory Medicine of St. Michael’s Hospital, Keenan Research Centre in the Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, and Department of Medicine, University of Toronto, and the Toronto Platelet Immunobiology Group, Toronto, Ontario, Canada

Hemolytic disease of the fetus and newborn can be effectively prevented by administration of anti-D to the mother. The administered IgG results in the attenuation of RBC-specific Ab production, a process termed Ab-mediated immune suppression (AMIS). Because in animal models of AMIS no major effect on T cell priming occurs, we hypothesized that the effect of the IgG on the immune system under AMIS conditions may involve a deficiency in B cell priming. We therefore challenged mice with either untreated RBCs or IgG-opsonized RBCs (AMIS) and assessed B cell priming. B cells from mice transfused with untreated RBCs, but not from mice treated under AMIS conditions, were primed as assessed by their ability to function as Ag-specific APCs to appropriate T cells. To our knowledge, this is the first report demonstrating that AMIS inhibits the appearance of Ag-primed RBC-specific B cells.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by grants from the Canadian Blood Services (to A.H.L.) and a Graduate Student Fellowship Award from the Canadian Blood Services (to D.B.).

² Address correspondence and reprint requests to Dr. Alan H. Lazarus, Transfusion Medicine Research, St. Michael’s Hospital, 30 Bond Street, Toronto, Ontario, Canada M5B 1W8. E-mail address: lazarusa{at}smh.toronto.on.ca

³ Abbreviations used in this paper: AMIS, Ab-mediated immune suppression; ASC, Ab-secreting cell.

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