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B-Dependent Pathway1School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan
Many cancers develop different means of escaping destruction by the immune system, such as resistance to Fas ligand (FasL)-Fas interaction-mediated apoptotic signals. Decoy receptor 3 (DcR3), a soluble receptor for FasL, is highly expressed in cancer cells and plays a significant role in immune suppression and tumor progression. However, how DcR3 expression is modulated is unclear. In this study, immunoprecipitation and ELISA using human pancreatic cancer cells showed the presence of high levels of DcR3 protein in AsPC-1 cells, but not in PANC-1 cells. Treatment with herbimycin A (a tyrosine kinase inhibitor), LY294002 or wortmannin (PI3K inhibitors), pyrrolidine dithiocarbamate (an NF-
B inhibitor), or AG1024 (an insulin-like growth factor-1 inhibitor) significantly reduced endogenous DcR3 levels in AsPC-1 cells. Furthermore, transfection of AsPC-1 cells with Akt or IB
dominant-negative plasmids also markedly reduced DcR3 levels. In contrast, 48-h transfection of PANC-1 cells with a constitutively active Akt induced DcR3 expression. Flow cytometry assays indicated that apoptosis was not seen in AsPC-1 cells incubated with soluble FasL or membrane-bound FasL, but was seen when DcR3 small interfering RNA-transfected AsPC-1 cells underwent the same treatment. In addition, PANC-1 cell incubation with conditioned medium from AsPC-1 cells transfected with dominant-negative Akt or IB plasmids or DcR3 small interfering RNA showed increased soluble FasL-mediated apoptosis compared with the control group. Our results show that insulin-like growth factor-1-induced activation of the PI3K/Akt/NF-B signaling pathway is involved in the modulation of endogenous DcR3 expression in AsPC-1 cells, and that reducing endogenous DcR3 levels increases FasL-induced apoptosis of human pancreatic cancer cells.
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1 This work was supported by the National Science Council of Taiwan (NSC96-2320-B-002-034; NSC97-2320-B-002-019-MY3).
2 Address correspondence and reprint requests to Dr. Chia-Ron Yang, School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan. E-mail address: cryang{at}ntu.edu.tw
3 Abbreviations used in this paper: DcR3, decoy receptor 3; Ct, cycle threshold; DN, dominant negative; FasL, Fas ligand; IGF, insulin-like growth factor; IKK, IB kinase; mFasL, membrane-bound FasL; PDTC, pyrrolidine dithiocarbamate; sFasL, soluble FasL; siRNA, small interfering RNA; SRB, sulforhodamine B.
4 The online version of this article contains supplemental material.
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  W. Wang, W. Sun, and L. Zhang Comment on "Decoy Receptor 3 Expression in AsPC-1 Human Pancreatic Adenocarcinoma Cells via the Phosphatidylinositol 3-Kinase-, Akt-, and NF-{kappa}B-Dependent Pathway" J. Immunol., August 15, 2009; 183(4): 2203 - 2203. [Full Text] [PDF]
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C.-R. Yang Response to Comment on "Decoy Receptor 3 Expression in AsPC-1 Human Pancreatic Adenocarcinoma Cells via the Phosphatidylinositol 3-Kinase-, Akt-, and NF-{kappa}B-Dependent Pathway" J. Immunol., August 15, 2009; 183(4): 2203 - 2204. [Full Text] [PDF]
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 C.-H. Ho, C.-L. Chen, W.-Y. Li, and C.-J. Chen Decoy receptor 3, upregulated by Epstein-Barr virus latent membrane protein 1, enhances nasopharyngeal carcinoma cell migration and invasion Carcinogenesis, August 1, 2009; 30(8): 1443 - 1451. [Abstract] [Full Text] [PDF]
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