| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Does Not Support Increased Expansion or Prevent Contraction of Antigen-Specific CD4 or CD8 T Cells following Listeria monocytogenes Infection1
* Department of Microbiology, Carver School of Medicine and
Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242; and
Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20824
Expression of IL-7R
(CD127) has been suggested as a major determinant in the survival of memory T cell precursors. We investigated whether constitutive expression of IL-7R on T cells increased expansion and/or decreased contraction of endogenous Ag-specific CD4 and CD8 T cells following infection with Listeria monocytogenes. The results indicate that constitutive expression of IL-7R alone was not enough to impart an expansion or survival advantage to CD8 T cells responding to infection, and did not increase memory CD8 T cell numbers over those observed in wild-type controls. Constitutive expression of IL-7R did allow for slightly prolonged expansion of Ag-specific CD4 T cells; however, it did not alter the contraction phase or protect against the waning of memory T cell numbers at later times after infection. Memory CD4 and CD8 T cells generated in IL-7R transgenic mice expanded similarly to wild-type T cells after secondary infection, and immunized IL-7R transgenic mice were fully protected against lethal bacterial challenge demonstrating that constitutive expression of IL-7R does not impair, or markedly improve memory/secondary effector T cell function. These results indicate that expression of IL-7R alone does not support increased survival of effector Ag-specific CD4 or CD8 T cells into the memory phase following bacterial infection.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by National Institutes of Health Grants AI42767, AI46653, and AI50073 (to J.T.H.), and American Cancer Society Grant PF-05-142-01-LIB (to J.S.H.).
2 Address correspondence and reprint requests to Dr. John T. Harty, Department of Microbiology, 3-512 Bowen Science Building, 51 Newton Road, Iowa City, IA 52242. E-mail address: john-harty{at}uiowa.edu
3 Abbreviations used in this paper: WT, wild type; Tg, transgenic; DP, double positive; p.i., post infection; ICS, intracellular cytokine staining; LLO, listeriolysin O.
Related articles in The JI:
This article has been cited by other articles:
|
|
 |
|
  S. L. Colpitts, N. M. Dalton, and P. Scott IL-7 Receptor Expression Provides the Potential for Long-Term Survival of Both CD62Lhigh Central Memory T Cells and Th1 Effector Cells during Leishmania major Infection J. Immunol., May 1, 2009; 182(9): 5702 - 5711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Mousavi, P. Soroosh, T. Takahashi, Y. Yoshikai, H. Shen, L. Lefrancois, J. Borst, K. Sugamura, and N. Ishii OX40 Costimulatory Signals Potentiate the Memory Commitment of Effector CD8+ T Cells J. Immunol., November 1, 2008; 181(9): 5990 - 6001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-H. Xue, X. Jing, J. Bollenbacher-Reilley, D.-M. Zhao, J. S. Haring, B. Yang, C. Liu, G. A. Bishop, J. T. Harty, and W. J. Leonard Targeting the GA Binding Protein {beta}1L Isoform Does Not Perturb Lymphocyte Development and Function Mol. Cell. Biol., July 1, 2008; 28(13): 4300 - 4309. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
