HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Milne, C. D. Articles by Paige, C. J. Search for Related Content PubMed PubMed Citation Articles by Milne, C. D. Articles by Paige, C. J. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Blood Thinners Stem Cells Hazardous Substances DB HEPARIN

Heparan Sulfate and Heparin Enhance ERK Phosphorylation and Mediate preBCR-Dependent Events during B Lymphopoiesis¹

Ontario Cancer Institute, University Health Network, Department of Immunology, University of Toronto, Toronto, Ontario, Canada

As B lineage cells develop, they interact with cells, proteins, and extracellular matrix components of the surrounding microenvironment. In vitro, one critical checkpoint for developing cells occurs as they lose responsiveness to IL-7. These cells require contact with either stromal cells or other B lineage cells to mature. Our results demonstrate that heparan sulfate and heparin are able to promote this transition when added exogenously to the culture system or when heparan sulfate-bearing cell lines are cocultured with primary B cell progenitors. Addition of heparan sulfate or heparin to LPS-stimulated cultures of primary B cell progenitors resulted in more IgM secreted compared with untreated cultures. Heparan sulfate has been reported to be a ligand for the pre-B cell receptor (preBCR). Extending this observation, we found that treatment of preBCR⁺ cells with heparan sulfate before anti-µ stimulation leads to increased phosphorylation of ERK1/2. Consequently, preBCR⁺ cells proliferate more in the presence of IL-7 and heparan sulfate, whereas preBCR^– cells are unaffected, suggesting that in these experiments, heparan sulfate is not directly affecting IL-7 activity. Heparin treatment of cultures induces many of the same biological effects as treatment with heparan sulfate, including elevated pERK levels in preBCR⁺ cells. However, heparin reduces the proliferation of cells expressing only the preBCR (opposed to both the preBCR and BCR) possibly due to internalization of the preBCR. Heparan sulfates are present on stromal cells and B lineage cells present in hemopoietic tissues and may provide stimulation to preB cells testing the signaling capacity of the preBCR.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by grants from the Terry Fox Foundation, the National Cancer Institute of Canada, and the Canadian Institute of Health Research.

² Address correspondence and reprint requests to Dr. Craig Milne, Ontario Cancer Institute, 8-105 Princess Margaret Hospital, Toronto, Ontario, Canada M5G 2M9. E-mail address: cdmilne{at}ualberta.ca

³ Abbreviations used in this paper: preBCR, pre-B cell receptor.

This article has been cited by other articles:

				C. Vettermann, K. Herrmann, C. Albert, E. Roth, M. R. Bosl, and H.-M. Jack A Unique Role for the {lambda}5 Nonimmunoglobulin Tail in Early B Lymphocyte Development J. Immunol., September 1, 2008; 181(5): 3232 - 3242. [Abstract] [Full Text] [PDF]