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The Central Memory CD4⁺ T Cell Population Generated during Leishmania major Infection Requires IL-12 to Produce IFN-¹

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104

Central memory CD4⁺ T cells provide a pool of lymph node-homing, Ag-experienced cells that are capable of responding rapidly after a secondary infection. We have previously described a population of central memory CD4⁺ T cells in Leishmania major-infected mice that were capable of mediating immunity to a secondary infection. In this study, we show that the Leishmania-specific central memory CD4⁺ T cells require IL-12 to produce IFN-, demonstrating that this population needs additional signals to develop into Th1 cells. In contrast, effector cells isolated from immune mice produced IFN- in vitro or in vivo in the absence of IL-12. In addition, we found that when central memory CD4⁺ T cells were adoptively transferred into IL-12-deficient hosts, many of the cells became IL-4 producers. These studies indicate that the central memory CD4⁺ T cell population generated during L. major infection is capable of developing into either Th1 or Th2 effectors. Thus, continued IL-12 production may be required to ensure the development of Th1 cells from this central memory T cell pool, a finding that has direct relevance to the design of vaccines dependent upon central memory CD4⁺ T cells.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by National Institutes of Health Grant AI35914. C.Z. is supported by the Irvington Institute Fellowship Program of the Cancer Research Institute.

² Address correspondence and reprint requests to Dr. Phillip Scott, University of Pennsylvania, 380 South University Avenue, Philadelphia, PA 19104-4539. E-mail address: pscott{at}vet.upenn.edu

³ Abbreviations used in this paper: Tcm, central memory T; dLN, draining lymph node; FTAg, freeze-thaw Ag; LACK, Leishmania homologue of receptor for activated kinase.

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The JI 2008 180: 7781-7782. [Full Text]  

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