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DNA Microarray Gene Expression Profile of Marginal Zone versus Follicular B Cells and Idiotype Positive Marginal Zone B Cells before and after Immunization with Streptococcus pneumoniae¹

Nicholas W. Kin^2,*, Dianna M. Crawford^2,3,, Jiabin Liu^4,, Timothy W. Behrens^5, and John F. Kearney^6,*

^* Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294; and Center for Immunology, Department of Medicine, University of Minnesota, Minneapolis, MN 55455

Marginal zone (MZ) B cells play an important role in the clearance of blood-borne bacterial infections via rapid T-independent IgM responses. We have previously demonstrated that MZ B cells respond rapidly and robustly to bacterial particulates. To determine the MZ-specific genes that are expressed to allow for this response, MZ and follicular (FO) B cells were sort purified and analyzed via DNA microarray analysis. We identified 181 genes that were significantly different between the two B cell populations. Ninety-nine genes were more highly expressed in MZ B cells while 82 genes were more highly expressed in FO B cells. To further understand the molecular mechanisms by which MZ B cells respond so rapidly to bacterial challenge, Id-positive and -negative MZ B cells were sort purified before (0 h) or after (1 h) i.v. immunization with heat-killed Streptococcus pneumoniae, R36A, and analyzed via DNA microarray analysis. We identified genes specifically up-regulated or down-regulated at 1 h following immunization in the Id-positive MZ B cells. These results give insight into the gene expression pattern in resting MZ vs FO B cells and the specific regulation of gene expression in Ag-specific MZ B cells following interaction with Ag.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by research funds from the National Institutes of Health Grant AI14782. N.W.K. is a recipient of a Training Grant Postdoctoral Fellowship Award from National Institutes of Health Grant T32 AI7051.

² N.W.K. and D.M.C. contributed equally to this work.

³ Current address: Amgen, Inc., Thousand Oaks, CA 91320.

⁴ Current address: Department of Anesthesia, Pennsylvania State College of Medicine, Milton S. Hershey Medical Center, Hershey, PA 17033.

⁵ Current address: Genentech, Inc., San Francisco, CA 94080.

⁶ Address correspondence and reprint requests to Dr. John F. Kearney, 410 Shelby Biomedical Research Building, 1825 University Boulevard, Birmingham, AL 35294. E-mail address: jfk{at}uab.edu

⁷ Abbreviations used in this paper: MZ, marginal zone; B6, C57BL/6; FO, follicular; S1P_{1, 3, or 4}, sphingosine-1-phosphate receptor type 1, 3, or 4; RGS, regulator of G protein; Tg, transgenic.