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Cutting Edge: Rapid Accumulation of Epidermal CCL27 in Skin-Draining Lymph Nodes following Topical Application of a Contact Sensitizer Recruits CCR10-Expressing T Cells¹

Victor Huang^*, Anke S. Lonsdorf^*, Lei Fang^*, Takashi Kakinuma, Vivian C. Lee^*, Emily Cha^*, Hong Zhang^*, Keisuke Nagao^*, Marzanna Zaleska, Waldemar L. Olszewski and Sam T. Hwang^2,*

^* Dermatology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892; and Medical Research Center, Polish Academy of Sciences, Warsaw, Poland

CC chemokine receptor 10 and its ligand, CCL27, are important components of T cell-mediated cutaneous immunity, but whether they influence lymph node (LN) homing by T cells is unknown. In this study, CCL27 protein was detected in skin-draining LN by Western blotting and ELISA although CCL27 mRNA transcripts were low. CCL27 protein was present at higher levels in skin-draining LN compared with gut-draining LN and spleen. A single topical treatment of mouse skin with the contact sensitizer 2,4-dinitro-1-fluorobenzene (DNFB) resulted in a 13-fold increase in CCL27 protein accumulation in skin-draining LN within 1 h and a 5-fold elevation in CCR10 mRNA (normalized to the T cell marker CD2) within 6 h. DNFB treatment also resulted in rapid depletion of 75% of CCL27 from the epidermis. In summary, we describe a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported in part by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research. A.S.L is supported by a National Institutes of Health-Deutsche Forschungsgemeinschaft Research Career Transition Award.

² Address correspondence and reprint requests to Dr. Sam T. Hwang, Dermatology Branch, Center for Cancer Research, National Cancer Institute, 10 Center Drive, Building 10, Room 12N238, Bethesda, MD 20892-1908. E-mail address: hwangs{at}mail.nih.gov

³ Abbreviations used in this paper: LN, lymph node; DNFB, 2,4-dinitro-1-fluorobenzene.