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Studies on the "Toxins" of Hemolytic Streptococci

IV. Comparison of Our Specific Toxin—true Scarlatinal Toxin—with the Purified Preparations Reported by Other Authors

From the Bacteriological Department (K. Ando), Hygienic Institute (Director S. Kanai), Dairen

Abstract

1. The specific toxin has been almost completely precipitated by the addition of 2 volumes of alcohol both from culture filtrate and from the broth culture itself, and easily obtained in a form of solution by simple saline extraction of this precipitate.

2. The specific toxin is not easily dialyzable.

3. No precipitation occurred when 2 volumes of alcohol were added to one volume of dialyzed culture filtrate, but abundant precipitate of the specific toxin was observed when in addition to alcohol a small amount of saturated salt solution was added to the dialyzed filtrate.

4. A small portion of the specific toxin may be precipitated by acid from a culture filtrate, dialyzed filtrate and crude specific toxin (solution of alcoholic precipitate).

5. When the alcoholic precipitate of specific toxin was dissolved in a small amount of saline solution, an insoluble part remained. The suspension and the washings from this insoluble part caused positive skin reactions on white pigs, while the washings from an acid precipitate of the culture filtrate or crude specific toxin gave no skin reaction on the same animals even in a higher concentration. Accordingly, the acid precipitate seems to contain the specific toxin not merely by adsorption, but perhaps in a form of chemical combination, probably, with nucleoprotein.